First detection of “Candidatus Rickettsia wissemanii” in Ornithodoros hasei (Schulze, 1935) (Acari: Argasidae) from Argentina
- Colombo, Valeria Carolina
- Montani, Maria Eugenia
- Pave, Romina Elizabeth
- Antoniazzi, Leandro Raúl
- Gamboa, Marcelo D.
- Fasano, Agustín A.
- Félix, Maria L.
- Nava, Santiago
- Venzal, José Manuel
DOIAbstract
The aim of this study was to assess the presence of Rickettsia in soft ticks (Acari: Argasidae) collected from insectivorous bats (Chiroptera) in Santa Fe province, Argentina. First, a subset of ticks were mounted in Hoyer’s medium to be determined by morphological characters and then confirmed by sequencing the mitochondrial 16S rRNA gene. Also ticks were processed by PCR assays using primers CS-78 and CS-323, which amplify a fragment of the Rickettsia spp. gltA gene. Positive ticks were subjected to a second PCR round with primers Rr190.70p and Rr190.701n of the spotted fever group rickettsiae ompA gene. A phylogenetic analysis was performed with Maximum-likelihood method, and the best fitting substitution models were determined with the ...
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